Identification of Lipase Producing Yeast Strains from Fresh Mango Fruit Juice

Ujunwa Felicia Nwachukwu* and Chizoba Jasmine Mbah

Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Nigeria

*Corresponding Author: Ujunwa Felicia Nwachukwu, Department of Applied Microbiology and Brewing, Faculty of Biological Sciences, Enugu State University of Science and Technology, Nigeria.

DOI: 10.64258/3069-7557.2025.103006

Submission Date: August 18, 2025

Published Date: August 28, 2025

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Abstract

The utilization of enzymes including lipase in the industry has intensified search for lipase-producing microorganisms from natural sources. This study aimed at identifying lipase producing yeast strains from mango fruit juice. The isolation of yeast strains from mango fruit juice was carried out using streaking method on potato dextrose agar (PDA) and was incubated at 300C for 48h. The yeast isolates were screened for lipolytic activity by point inoculation on tributyrin agar. The yeast strains which had lipolytic activity were identified and characterized phenotypically by morphological characterization, Gram staining, biochemical tests, sugar fermentation and molecular approaches including DNA extraction, electrophoresis, gene amplification and sequencing. The obtained results revealed that all isolated yeast strains hydrolyzed the tributyrin in the agar by recording mean zone of hydrolysis of ±30mm. The phenotypic and molecular characterizations revealed the lipolytic yeast strains as Pichia kudriavzevii FJIB1- FJIB3. Pichia kudriavzevii FJIB1- FJIB3 had 99.35% pairwise similarity with Pichia kudriavzevii strain KBP: YE-1321 which has NCBI accession number MW856016.1. These findings highlight the potential of indigenous Pichia kudriavzevii FJIB1- FJIB3 from mango juice as cost-effective sources of lipases which can be utilized for lipase production in relevant industries including food.

Keywords

Pichia kudriavzevii, Mango Juice, Isolation, Identification, Lipase

Citation

Ujunwa FN, Chizoba Jasmine (2025) Identification of Lipase Producing Yeast Strains from Fresh Mango Fruit Juice. Ann Biotech & Biomed Sci 1(2): 1-6.